Quantitative real-time polymerase chain response detection of BK virus utilizing labeled primers.
BACKGROUND
BK virus infections amongst immunocompromised sufferers are related to illness of the kidney or urinary bladder. Excessive viral hundreds, decided by quantitative polymerase chain response (PCR), have been correlated with medical illness.
OBJECTIVE
To develop and consider a novel technique for real-time PCR detection and quantification of BK virus utilizing labeled primers.
METHODS
Affected person specimens (n = 54) included 17 plasma, 12 entire blood, and 25 urine samples. DNA was extracted utilizing the MagNA Pure LC Complete Nucleic Acid Isolation Package (Roche Utilized Science, Indianapolis, Indiana); pattern eluate was PCR-amplified utilizing the labeled primer PCR technique. Outcomes have been in contrast with these of a user-developed quantitative real-time PCR technique (fluorescence resonance power switch probe hybridization).
Ferret IgG (Control, non-immune, isotype control), semi-pure for ELISA Kit
RESULTS
Labeled primer PCR detected lower than 10 copies per response and confirmed quantitative linearity from 10(1) to 10(7) copies per response. Analytical specificity of labeled primer PCR was 100%. With medical samples, labeled primer PCR demonstrated a development towards improved sensitivity in contrast with the reference technique. Quantitative assay comparability confirmed an R(2) worth of 0.96 between the two assays.
Skunk IgG, semi-pure for ELISA (Control, non-immune, isotype control)
CONCLUSIONS
Actual-time PCR utilizing labeled primers is very delicate and particular for the quantitative detection of BK virus from quite a lot of medical specimens. These knowledge reveal the applicability of labeled primer PCR for quantitative viral detection and provide a simplified technique that removes the necessity for separate oligonucleotide probes.

NATtrol HIV Type 1 Strain: IIIB Linearity Panel (6 X 0.25 mL) |
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Better Bradford Protein Assay Kit (Improved linearity) |
SK3041 |
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1000Assays, 1000preps |
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Zika Virus Seroconversion Panel (7 X 0.5mL) |
KZMC047 |
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Mouse antibody for BK virus VP1 |
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Description: This is purified Mouse monoclonal antibody against BK virus VP1 for WB, ELISA. |
Mouse antibody for BK virus VP1 |
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Description: This is purified Mouse monoclonal antibody against BK virus VP1 for WB, ELISA. |
Frozen Tissue Section Panel - Mouse Whole Brain Segmentation Panel |
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VAHTS Target Capture Core Exome Panel |
NC001-01 |
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VAHTS Target Capture Core Exome Panel |
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96 rxns |
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Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel I, 5 tissue spots |
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5 slides |
EUR 363 |
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5 slides |
EUR 363 |
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5 slides |
EUR 363 |
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel IV, 5 tissue spots |
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5 slides |
EUR 363 |
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel V, 5 tissue spots |
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5 slides |
EUR 363 |
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VI, 5 tissue spots |
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EUR 363 |
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel I, 8 tissue spots |
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EUR 565 |
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5 slides |
EUR 565 |
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EUR 565 |
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5 slides |
EUR 565 |
Paraffin Tissue Section Panel - Human Tumor, Different Tumor Type, Different Donors, Panel VI, 8 tissue spots |
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Histone H4 Acetylation Antibody Panel Pack |
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EpiGentek |
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B6/C57J Immunoglobulin Panel Kit (OKSB00004) |
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EUR 482.4 |
Description: Description of target: ;Species reactivity: ;Application: ELISA;Assay info: ;Sensitivity: |
DiscoveryProbe? Apoptosis-related Compounds Panel |
L1002-5 |
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5 mg/well |
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NATtrol BK Virus External Run Control, Low (6 X 1 mL) |
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C10005 |
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Lung carcinoma (multi-tissue combined panel |
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L1013-5 |
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EUR 7617.6 |
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Histone H3K27 Methylation Antibody Panel Pack |
C10007 |
EpiGentek |
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Improvement of a BK virus real-time quantitative assay utilizing the bioMérieux analyte-specific reagents in plasma specimens.
-
METHODS
This examine evaluated the efficiency of the analyte-specific reagent (ASR) BKV primers r-gene and probe r-gene reagents (bioMérieux, Marcy l’Étoile, France) quickly to turn into out there on the US marketplace for accuracy, linearity, precision, analytical sensitivity, specificity, and correlation with the Qiagen (Germantown, MD) BKV ASR check utilizing business materials and affected person plasma samples.
ADAPTERS FOR 5/7ML TUBES IN 50ML ROTOR,6/CS
-
RESULTS
The assay was linear from 204 to three.92 million (2.31-6.6 log10) DNA copies/mL (coefficient of dedication: R(2) =0.999). A dilution sequence demonstrated limits of detection and quantitation of two.14 log10 and a couple of.30 log10 copies/mL (95% hit fee detection), respectively. Interrun precision was extremely reproducible, with coefficients of variance starting from 2.2% to six.0%. A comparability of 34 matched samples confirmed a great settlement (R(2) = 0.87) between the bioMérieux BKV laboratory check and the Qiagen BKV ASR assay outcomes, with a median adverse bias (-0.28 log10 copies/mL).
- Human polyomavirus BK (BKV) is more and more acknowledged as an opportunistic pathogen in transplant recipients. The intention of this work was to judge the artus(®) BK Virus QS-RGQ assay on the QIAsymphony RGQ system in entire blood (WB) samples (exams carried out in an off-label capability) in keeping with totally different BKV genotypes by comparability with a laboratory-developed assay. BKV hundreds have been measured in 111 WB samples and BKV genotype was decided by sequencing the full-length VP1 gene.
- The artus(®) assay exhibited a restrict of detection of 77copies/mL, a linearity vary from 3.Zero to six.0log10copies/mL, intra-assay and inter-assay coefficients of variation starting from 0.65% to five.18%. Relating to BKV quantitation, artus(®) and laboratory-developed assays have been extremely correlated (Spearman correlation coefficient Rho=0.79; P<0.0001) with a superb general settlement (96.4%) and no vital quantitative distinction in keeping with Bland-Altman evaluation (imply distinction: -0.34log10copies/mL).
- The outcomes didn’t present any affect of BKV genotype on BKV quantitation by the artus(®) assay, besides a possible underquantitation of BKV subtype Ia which deserves additional affirmation. In conclusion, the QIAsymphony RGQ system seems to be acceptable for the quantitation of BKV load in WB samples.
Adenovirus antibody |
10-A02B |
Fitzgerald |
200 ug |
EUR 337.2 |
Description: Mouse monoclonal Adenovirus antibody |
Adenovirus antibody |
10-A02C |
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EUR 273.6 |
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Fitzgerald |
1 mg |
EUR 636 |
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200 ug |
EUR 268.8 |
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EUR 961.2 |
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EUR 1111.2 |
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EUR 710.4 |
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0.2 mg |
EUR 1345.2 |
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abx021524-1mg |
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1 mg |
EUR 1220.4 |
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Adeno-SV40 Adenovirus |
G210 |
ABM |
250 ul |
EUR 1216.8 |
Adenovirus RT PCR kit |
RTq-H501-100D |
Bioingentech |
100T |
EUR 860.4 |
Adenovirus RT PCR kit |
RTq-H501-150D |
Bioingentech |
150T |
EUR 969.6 |
Adenovirus RT PCR kit |
RTq-H501-50D |
Bioingentech |
50T |
EUR 717.6 |
Adenovirus antibody (HRP) |
60-A02 |
Fitzgerald |
1 ml |
EUR 494.4 |
Description: Goat polyclonal Adenovirus antibody (HRP) conjugated |
Adenovirus E1A antibody |
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EUR 262.8 |
Description: Sheep polyclonal Adenovirus E1A antibody |
CRISPR / hCas9 Adenovirus |
AVP010 |
GenTarget |
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Adenovirus (ADV) Antibody |
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1 mg |
EUR 994.8 |
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Adenovirus (ADV) Antibody |
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1 ml |
EUR 610.8 |
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Adenovirus (ADV) Antibody |
abx414317-1ml |
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1 ml |
EUR 794.4 |
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Adenovirus antibody (FITC) |
60C-CR1238GF |
Fitzgerald |
1 ml |
EUR 435.6 |
Description: Goat polyclonal Adenovirus antibody (FITC) conjugated |
Adenovirus antibody (FITC) |
61-A02A |
Fitzgerald |
100 ug |
EUR 429.6 |
Description: Mouse monoclonal Adenovirus antibody (FITC) |
Adenovirus antibody (FITC) |
60-A01 |
Fitzgerald |
1 ml |
EUR 440.4 |
Description: Goat polyclonal Adenovirus antibody (FITC) conjugated |
Adenovirus protein (Mouse) |
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1 mg |
EUR 289.2 |
Description: Purified native Adenovirus protein (Mouse) |
Adenovirus Antibody (FITC) |
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1 ml |
EUR 777.6 |
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abx021508-1mg |
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1 mg |
EUR 1496.4 |
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Fowl Adenovirus PCR kit |
PCR-V040-48D |
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50T |
EUR 543.6 |
Fowl Adenovirus PCR kit |
PCR-V040-96D |
Bioingentech |
100T |
EUR 686.4 |
RFP expression Adenovirus |
AVP001 |
GenTarget |
1x109 IFU/ml x 200ul |
EUR 418.8 |
Description: pre-made RFP expression adenovirus, provided in DMEM medium. |
CFP expression Adenovirus |
AVP002 |
GenTarget |
1x109 IFU/ml x 200ul |
EUR 418.8 |
Description: pre-made CFP expression adenovirus, provided in DMEM medium. |
Luciferase / GFP Adenovirus |
AVP004 |
GenTarget |
1x109 IFU/ml x 200ul |
EUR 418.8 |
Description: pre-made Luciferase / GFP expression adenovirus, provided in DMEM medium. |
Luciferase / RFP Adenovirus |
AVP005 |
GenTarget |
1x109 IFU/ml x 200ul |
EUR 418.8 |
Description: pre-made Luciferase / RFP expression adenovirus, provided in DMEM medium. |
GFP expression Adenovirus |
AVP011 |
GenTarget |
1x109 IFU/ml x 200ul |
EUR 418.8 |
Description: pre-made GFP expression adenovirus, provided in DMEM medium. |
YFP expression Adenovirus |
AVP012 |
GenTarget |
1x109 IFU/ml x 200ul |
EUR 418.8 |
Description: pre-made YFP expression adenovirus, provided in DMEM medium. |
BFP expression Adenovirus |
AVP017 |
GenTarget |
1x109 IFU/ml x 200ul |
EUR 418.8 |
Description: pre-made BFP expression adenovirus, provided in DMEM medium. |
Adenovirus Hexon Antibody |
abx023633-1mg |
Abbexa |
1 mg |
EUR 794.4 |
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Adenovirus Hexon Antibody |
abx023661-1mg |
Abbexa |
1 mg |
EUR 718.8 |
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Adenovirus Hexon Antibody |
abx023662-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx023854-1mg |
Abbexa |
1 mg |
EUR 718.8 |
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Adenovirus Hexon Antibody |
abx023856-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx023857-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx023858-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx023872-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx024001-1mg |
Abbexa |
1 mg |
EUR 1212 |
|
Adenovirus Hexon Antibody |
abx024002-1mg |
Abbexa |
1 mg |
EUR 1212 |
|
Adenovirus Hexon Antibody |
abx024003-1mg |
Abbexa |
1 mg |
EUR 1212 |
|
Adenovirus Hexon Antibody |
abx021514-1mg |
Abbexa |
1 mg |
EUR 1011.6 |
|
Adenovirus Hexon Antibody |
abx021516-1mg |
Abbexa |
1 mg |
EUR 1011.6 |
|
Adenovirus Hexon Antibody |
abx021518-1mg |
Abbexa |
1 mg |
EUR 1220.4 |
|
Adenovirus Hexon Antibody |
abx021519-1mg |
Abbexa |
1 mg |
EUR 1111.2 |
|
Adenovirus Hexon Antibody |
abx021520-1mg |
Abbexa |
1 mg |
EUR 878.4 |
|
Adenovirus Hexon Antibody |
abx021523-1mg |
Abbexa |
1 mg |
EUR 878.4 |
|
Adenovirus Hexon Antibody |
abx021525-1mg |
Abbexa |
1 mg |
EUR 878.4 |
|
Adenovirus Hexon Antibody |
abx120000-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx120001-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx120002-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx120003-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx120004-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx120005-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx120006-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus Hexon Antibody |
abx120007-1mg |
Abbexa |
1 mg |
EUR 718.8 |
|
Adenovirus IgA ELISA kit |
55R-IB79201 |
Fitzgerald |
96 wells |
EUR 379.2 |
Description: ELISA kit for the detection of Adenovirus IgA in the research laboratory |
Adenovirus IgG ELISA kit |
55R-IB79202 |
Fitzgerald |
96 wells |
EUR 396 |
Description: ELISA kit for the detection of Adenovirus IgG in the research laboratory |
Adenovirus IgM ELISA kit |
55R-IB79203 |
Fitzgerald |
96 wells |
EUR 382.8 |
Description: ELISA kit for the detection of Adenovirus IgM in the research laboratory |
Adenovirus IgG ELISA Kit |
DEIA309 |
Creative Diagnostics |
96T |
EUR 780 |
Description: The Adenovirus IgG Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgG antibodies against Adenovirus in serum and plasma. |
Adenovirus IgA ELISA Kit |
DEIA310 |
Creative Diagnostics |
96T |
EUR 780 |
Description: The Adenovirus IgA Antibody ELISA Test Kit has been designed for the the detection and the quantitative determination of specific IgA antibodies against Adenovirus in serum and plasma. |
Adenovirus IgM ELISA Kit |
DEIA311 |
Creative Diagnostics |
96T |
EUR 780 |
Description: The Adenovirus IgM Antibody ELISA Test Kit has been designed forthe the detection and the quantitative determination of specific IgM antibodies against Adenovirus in serum and plasma. |
Mouse Adenovirus PCR kit |
PCR-V366-48D |
Bioingentech |
50T |
EUR 543.6 |
Mouse Adenovirus PCR kit |
PCR-V366-96D |
Bioingentech |
100T |
EUR 686.4 |
Adenovirus antibody (biotin) |
60C-CR1238GB |
Fitzgerald |
1 ml |
EUR 435.6 |
Description: Goat polyclonal Adenovirus antibody (biotin) conjugated |
GFP Recombinant Adenovirus |
ADV-004 |
Cell Biolabs |
50 ?L |
EUR 1069.2 |
Description: Premade recombinant adenovirus containing the GFP gene |
Cre Recombinant Adenovirus |
ADV-005 |
Cell Biolabs |
50 ?L |
EUR 1069.2 |
Description: Premade recombinant adenovirus containing the Cre gene |
Adenovirus 40 & 41 Antibody |
abx021513-1mg |
Abbexa |
1 mg |
EUR 710.4 |
|
Falcon adenovirus PCR kit |
PCR-V017-96D |
Bioingentech |
100T |
EUR 686.4 |