Improved strategies to measure finish merchandise of nitric oxide in organic fluids: nitrite, nitrate, and S-nitrosothiols.
- The purpose of this examine was to check and enhance commonplace strategies to find out nitrite (NO2-), nitrate (NO3-) and S-nitrosothiol (RSNO) ranges in cell tradition supernatants, sera, and urine. We modified the standard Griess response by changing sulfanilamide with dapsone (4,4′-diamino–diphenylsulfone) and in contrast the NO2– ranges in our examine samples with a commercially out there NO2- assay package.
- Our modification, together with ultrafiltration of the samples, resulted in an enhanced sensitivity to measure NO2- all the way down to 0.2 microM. The detection restrict was additional improved to 0.02 microM when NO2- was recognized by the fluorochrome 2,3-diaminonaphthalene (DAN). To measure the steady finish product NO3– by the Griess response or the DAN technique, this anion have to be diminished to NO2-. We in contrast the capability of bacterial nitrate reductase with the decreasing metallic cadmium to transform NO3- to NO2-.
- After discount, NO2- ranges have been decided both by the DAN technique or by our modified Griess response. We discovered that there was a excessive correlation (r2 = 0.998) in complete NO2- concentrations within the examine samples utilizing each strategies for decreasing NO3- to NO2-. The simultaneous dedication of NO2- and NO3- was achieved through the use of anion-exchange chromatography (HPLC; Polyspher IC AN-1 column).
Ferret IgG (Control, non-immune, isotype control), semi-pure for ELISA
- The detection restrict of this assay for every anion is 0.5 microM, and it may be utilized equally nicely to sera, urine, and tradition media. We additionally tailored the DAN technique to find out RSNO ranges in our examine samples. Utilizing this strategy, we have been in a position to measure RSNO ranges all the way down to 0.15 microM.
- As outcome we found that RSNO ranges have been markedly elevated in urine from septic sufferers and in supernatants from cytokine-stimulated human tumor cell traces. L-Citrulline, a coproduct of NO biosynthesis, was measured utilizing a colorimetric assay with a sensitivity restrict of three.Zero microM. Elevated L-citrulline ranges in media from traditiond cells, however not in sera or urine, correlated with elevated NO manufacturing.
Goat IgG (-ve control for flow cytometry) (isotype control)
- Though all strategies studied have been appropriate for quantifying finish merchandise of NO in organic fluids and media, using bacterial reductase and the modified Griess response proved profitable to supply the best sensitivity and linear vary for routine measurements of NO2- and NO3-.
ALT. THIOGLYCOLLATE MED. |
|||
| A01-103-2Kg | Alphabiosciences | 2 Kg | Ask for price |
ALT. THIOGLYCOLLATE MED. |
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| A01-103-500g | Alphabiosciences | 500 g | Ask for price |
Bouin's Fluid |
|||
| BNF125 | ScyTek Laboratories | 125 ml | EUR 63 |
Bouin's Fluid |
|||
| BNF3800 | ScyTek Laboratories | 1 Gal. | EUR 178 |
Bouin's Fluid |
|||
| BNF500 | ScyTek Laboratories | 500 ml | EUR 80 |
Bouin's Fluid |
|||
| BNF999 | ScyTek Laboratories | 1000 ml | EUR 98 |
Michel's Transport Fluid |
|||
| MTF-10000 | ScyTek Laboratories | 10 L | EUR 762 |
Michel's Transport Fluid |
|||
| MTF-20000 | ScyTek Laboratories | 20 L | EUR 1340 |
Michel's Transport Fluid |
|||
| MTF500 | ScyTek Laboratories | 500 ml | EUR 98 |
Michel's Transport Fluid |
|||
| MTF999 | ScyTek Laboratories | 1000 ml | EUR 127 |
SOF Synthetic Oviduct Fluid. |
|||
| IVL05-100ML | Caisson Labs | 100 ml | EUR 78 |
|
Description: Synthetic Oviduct Fluid. |
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SOF Synthetic Oviduct Fluid. |
|||
| IVL05-6X100ML | Caisson Labs | 6 x 100 ml | EUR 151 |
|
Description: Synthetic Oviduct Fluid. |
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HE4, Human ascites fluid |
|||
| P1438-100 | Biovision | EUR 620 | |
HE4, Human ascites fluid |
|||
| P1438-500 | Biovision | EUR 2839 | |
MagSi-DNA Body Fluid |
|||
| MDKT00140096 | AMSBIO | 96 preps | EUR 185 |
MagSi-DNA Body Fluid |
|||
| MDKT00140960 | AMSBIO | 10 x 96 preps | EUR 1370 |
20 x Synovial Fluid Solution |
|||
| GR103059 | Genorise Scientific | 100 mL | EUR 70 |
CA-125, Human Ascites Fluid |
|||
| P1446-10 | Biovision | EUR 294 | |
M9CA Medium |
|||
| SD7025 | Bio Basic | 250g | EUR 71.75 |
YM Medium |
|||
| SD7031 | Bio Basic | 250g | EUR 70.45 |
TYGPN Medium |
|||
| SD7032 | Bio Basic | 250g | EUR 70.01 |
M63 Medium |
|||
| SD7033 | Bio Basic | 250g | EUR 71.75 |
M9 Medium |
|||
| SD7024 | Bio Basic | 250g | EUR 71.75 |
A Medium |
|||
| DJ1018 | Bio Basic | 100g | EUR 84.8 |
NeuroProgenitor Medium |
|||
| NM42400 | Neuromics | 125 ml | EUR 304 |
Heller's Medium |
|||
| CP014-010 | GenDepot | 10X1L | EUR 99 |
Heller's Medium |
|||
| CP014-500 | GenDepot | 50L | EUR 126 |
Hoagland's Medium |
|||
| CP015-010 | GenDepot | 10X1L | EUR 99 |
Hoagland's Medium |
|||
| CP015-500 | GenDepot | 50L | EUR 126 |
Advanced Medium |
|||
| C0003-04 | Addexbio | RT 500 mL Bottle | EUR 103 |
Medium 199 |
|||
| C0012-01 | Addexbio | RT 500 mL Bottle | EUR 105 |
DC MEDIUM |
|||
| D04-117-10kg | Alphabiosciences | 10 kg | EUR 1579 |
DC MEDIUM |
|||
| D04-117-2kg | Alphabiosciences | 2kg | EUR 382 |
DC MEDIUM |
|||
| D04-117-500g | Alphabiosciences | 500 g | EUR 140 |
HLP MEDIUM |
|||
| H08-107-10kg | Alphabiosciences | 10 kg | EUR 2278 |
HLP MEDIUM |
|||
| H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 534 |
HLP MEDIUM |
|||
| H08-107-500g | Alphabiosciences | 500 g | EUR 182 |
EC MEDIUM |
|||
| E05-100-10kg | Alphabiosciences | 10 kg | EUR 814 |
EC MEDIUM |
|||
| E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 216 |
EC MEDIUM |
|||
| E05-100-500g | Alphabiosciences | 500 g | EUR 95 |
COLIFORM MEDIUM |
|||
| C03-127-10kg | Alphabiosciences | 10 kg | EUR 1324 |
COLIFORM MEDIUM |
|||
| C03-127-2kg | Alphabiosciences | 2kg | EUR 327 |
COLIFORM MEDIUM |
|||
| C03-127-500g | Alphabiosciences | 500 g | EUR 125 |
SIM MEDIUM |
|||
| S19-110-10kg | Alphabiosciences | 10 kg | EUR 1021 |
SIM MEDIUM |
|||
| S19-110-2kg | Alphabiosciences | 2kg | EUR 261 |
SIM MEDIUM |
|||
| S19-110-500g | Alphabiosciences | 500 g | EUR 107 |
SOB MEDIUM |
|||
| S19-124-10kg | Alphabiosciences | 10 kg | EUR 965 |
SOB MEDIUM |
|||
| S19-124-2kg | Alphabiosciences | 2kg | EUR 249 |
SOB MEDIUM |
|||
| S19-124-500g | Alphabiosciences | 500 g | EUR 104 |
Exosome Isolation kit (forHuman Body Fluid) |
|||
| P121 | 101Bio | - | Ask for price |
Exosome Isolation kit (forHuman Body Fluid) |
|||
| P121L | 101Bio | - | Ask for price |
Exosome Isolation kit (forHuman Body Fluid) |
|||
| P121S | 101Bio | - | Ask for price |
Cancer Antigen 125 Antigen (Ascites Fluid) |
|||
| VAng-Cr3870-10kU | Creative Biolabs | 10 kU | EUR 944 |
|
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE. |
|||
Insect Cell Medium: TNM-FH Insect Culture Medium |
|||
| ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Serum-Free Insect Culture Medium |
|||
| ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
|||
| ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
H Medium Broth |
|||
| DJ1019 | Bio Basic | 100g | EUR 60.88 |
Agarose, medium EEO |
|||
| GE4750-100G | Glentham Life Sciences | 100 g | EUR 166 |
Agarose, medium EEO |
|||
| GE4750-500G | Glentham Life Sciences | 500 g | EUR 429 |
Schwann Growth Medium |
|||
| SGM001 | Neuromics | 500 ml | EUR 244 |
Hepatocyte Maintenance Medium |
|||
| HM42600 | Neuromics | 100 ml | EUR 409 |
Neuron Growth Medium |
|||
| HNM001 | Neuromics | 500 ml | EUR 331 |
MCDB 131 Medium |
|||
| CM034-050 | GenDepot | 500ml | EUR 85 |
MCDB 131 Medium |
|||
| CM034-300 | GenDepot | 6x500ml | EUR 165 |
MCDB 131 Medium |
|||
| CM034-310 | GenDepot | 10x500ml | EUR 219 |
MCDB 131 Medium |
|||
| CM034-320 | GenDepot | 20x500ml | EUR 340 |
MCDB 131 Medium |
|||
| CM034-350 | GenDepot | 50x500ml | EUR 585 |
Williams' Medium E |
|||
| CM063-050 | GenDepot | 500ml | EUR 91 |
Williams' Medium E |
|||
| CM063-300 | GenDepot | 6x500ml | EUR 266 |
Williams' Medium E |
|||
| CM063-310 | GenDepot | 10x500ml | EUR 313 |
Williams' Medium E |
|||
| CM063-320 | GenDepot | 20x500ml | EUR 604 |
Williams' Medium E |
|||
| CM063-350 | GenDepot | 50x500ml | EUR 975 |
Cereal Crops Medium |
|||
| CP003-005 | GenDepot | 5L | EUR 113 |
Cereal Crops Medium |
|||
| CP003-010 | GenDepot | 10X1L | EUR 169 |
Kao Michayluk Medium |
|||
| CP017-010 | GenDepot | 10X1L | EUR 99 |
Kao Michayluk Medium |
|||
| CP017-500 | GenDepot | 50L | EUR 126 |
Orchid Maintenance Medium |
|||
| CP037-010 | GenDepot | 10X1L | EUR 109 |
Orchid Maintenance Medium |
|||
| CP037-500 | GenDepot | 50L | EUR 138 |
Woody Plants Medium |
|||
| CP040-010 | GenDepot | 10X1L | EUR 101 |
Woody Plants Medium |
|||
| CP040-500 | GenDepot | 50L | EUR 126 |
Recovery Medium - 6x12mL |
|||
| 1711 | Intact Genomics | 4/PK | EUR 128 |
EverBrite Mounting Medium |
|||
| 23001 | Biotium | 10mL | EUR 178 |
|
Description: Minimum order quantity: 1 unit of 10mL |
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AddexBio Cryopreservation Medium |
|||
| C0002-20mL | Addexbio | RT vial | EUR 104 |
Aqueous Mounting Medium |
|||
| AR1018 | BosterBio | 10mL X5 (Enough for 800-1200 assays) | EUR 80 |
Antifade Mounting Medium |
|||
| AR1109 | BosterBio | 10mL (for 1000 assays) | EUR 65 |
RPMI 1640 Medium |
|||
| abx098879-500ml | Abbexa | 500 ml | EUR 175 |
FluoreGuard Mounting Medium |
|||
| FMM030 | ScyTek Laboratories | 30 ml | EUR 96 |
FluoreGuard Mounting Medium |
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| FMM060 | ScyTek Laboratories | 60 ml | EUR 116 |
FluoreGuard Mounting Medium |
|||
| FMM500 | ScyTek Laboratories | 500 ml | EUR 327 |
Monitoring of the uptake and metabolism of aminooxy analogues of polyamines in cultured cells by high-performance liquid chromatography.
- A high-performance liquid chromatographic technique for the dedication of polyamines and their aminooxy analogues is described. Oxime derivatization with a ketone is used to guard the aminooxy group throughout post-column response with o-phthalaldehyde. The quantity of the polyamines and of the oximes of their aminooxy analogues might be decided concurrently in traditiond cells and cell tradition media.
- The restrict of detection is 20-30 pmol, and the response of the fluorescence detection is linear as much as Four nmol. The separation of the aminooxy analogues from the naturally occurring polyamines might be various through the use of totally different ketones for oxime formation. The strategy was used to measure the soundness of aminooxy analogues of putrescine (1-aminooxy-3-aminopropane) and spermidine [N-(2-aminooxyethyl)-1,4-diaminobutane and 1-aminooxy-3-N-(3-aminopropyl)aminopropane] in cell tradition media and the uptake into traditiond child hamster kidney (BHK21/C13) cells.
Saccharomyces Cerevisiae Host Cell Proteins positive control (10 ug/ml)
- The prognosis of human herpesvirus 6 (HHV-6) an infection represents a posh difficulty as a result of essentially the most broadly used diagnostic instruments, resembling immunoglobulin G antibody titer dedication and qualitative DNA PCR with blood cells, are unable to tell apart between latent (clinically silent) and lively (usually clinically related) an infection.
Llama IgG (Control, non-immune, isotype control, ELISA grade)
- We’ve developed a brand new, extremely delicate, quantitative PCR assay for the correct measurement of HHV-6 DNA in tissue-derived cell suspensions and physique fluids. The check makes use of a 5′ nuclease, fluorogenic assay mixed with real-time detection of PCR amplification merchandise with the ABI PRISM 7700 sequence detector system. The sensitivity of this technique is the same as the sensitivity of a nested PCR protocol (decrease detection restrict,
FLUID THIOGLYCOLLATE MEDIUM |
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| F06-101-500g | Alphabiosciences | 500 g | EUR 94 |
SABOURAUD DEXTROSE AGAR |
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| S19-100-10kg | Alphabiosciences | 10 kg | EUR 750 |
SABOURAUD DEXTROSE AGAR |
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| S19-100-2Kg | Alphabiosciences | 2 Kg | EUR 202 |
SABOURAUD DEXTROSE AGAR |
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| S19-100-500g | Alphabiosciences | 500 g | EUR 91 |
SABOURAUD DEXTROSE BROTH |
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| S19-129-10kg | Alphabiosciences | 10 kg | EUR 1014 |
SABOURAUD DEXTROSE BROTH |
|||
| S19-129-2kg | Alphabiosciences | 2kg | EUR 260 |
SABOURAUD DEXTROSE BROTH |
|||
| S19-129-500g | Alphabiosciences | 500 g | EUR 107 |
SABOURAUD DEXTROSE AGAR, EMMONS |
|||
| S19-102-10kg | Alphabiosciences | 10 kg | EUR 1148 |
SABOURAUD DEXTROSE AGAR, EMMONS |
|||
| S19-102-2kg | Alphabiosciences | 2kg | EUR 289 |
SABOURAUD DEXTROSE AGAR, EMMONS |
|||
| S19-102-500g | Alphabiosciences | 500 g | EUR 115 |
Bouin's Fluid |
|||
| BNF125 | ScyTek Laboratories | 125 ml | EUR 63 |
Bouin's Fluid |
|||
| BNF3800 | ScyTek Laboratories | 1 Gal. | EUR 178 |
Bouin's Fluid |
|||
| BNF500 | ScyTek Laboratories | 500 ml | EUR 80 |
Bouin's Fluid |
|||
| BNF999 | ScyTek Laboratories | 1000 ml | EUR 98 |
SABOURAUD DEXTROSE AGAR W/CHLORAMPHENICOL |
|||
| S19-100C-10kg | Alphabiosciences | 10 kg | EUR 1020 |
SABOURAUD DEXTROSE AGAR W/CHLORAMPHENICOL |
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| S19-100C-2kg | Alphabiosciences | 2kg | EUR 261 |
SABOURAUD DEXTROSE AGAR W/CHLORAMPHENICOL |
|||
| S19-100C-500g | Alphabiosciences | 500 g | EUR 107 |
Michel's Transport Fluid |
|||
| MTF-10000 | ScyTek Laboratories | 10 L | EUR 762 |
Michel's Transport Fluid |
|||
| MTF-20000 | ScyTek Laboratories | 20 L | EUR 1340 |
Michel's Transport Fluid |
|||
| MTF500 | ScyTek Laboratories | 500 ml | EUR 98 |
Michel's Transport Fluid |
|||
| MTF999 | ScyTek Laboratories | 1000 ml | EUR 127 |
SOF Synthetic Oviduct Fluid. |
|||
| IVL05-100ML | Caisson Labs | 100 ml | EUR 78 |
|
Description: Synthetic Oviduct Fluid. |
|||
SOF Synthetic Oviduct Fluid. |
|||
| IVL05-6X100ML | Caisson Labs | 6 x 100 ml | EUR 151 |
|
Description: Synthetic Oviduct Fluid. |
|||
HE4, Human ascites fluid |
|||
| P1438-100 | Biovision | EUR 620 | |
HE4, Human ascites fluid |
|||
| P1438-500 | Biovision | EUR 2839 | |
MagSi-DNA Body Fluid |
|||
| MDKT00140096 | AMSBIO | 96 preps | EUR 185 |
MagSi-DNA Body Fluid |
|||
| MDKT00140960 | AMSBIO | 10 x 96 preps | EUR 1370 |
20 x Synovial Fluid Solution |
|||
| GR103059 | Genorise Scientific | 100 mL | EUR 70 |
CA-125, Human Ascites Fluid |
|||
| P1446-10 | Biovision | EUR 294 | |
M9CA Medium |
|||
| SD7025 | Bio Basic | 250g | EUR 71.75 |
YM Medium |
|||
| SD7031 | Bio Basic | 250g | EUR 70.45 |
TYGPN Medium |
|||
| SD7032 | Bio Basic | 250g | EUR 70.01 |
M63 Medium |
|||
| SD7033 | Bio Basic | 250g | EUR 71.75 |
M9 Medium |
|||
| SD7024 | Bio Basic | 250g | EUR 71.75 |
A Medium |
|||
| DJ1018 | Bio Basic | 100g | EUR 84.8 |
NeuroProgenitor Medium |
|||
| NM42400 | Neuromics | 125 ml | EUR 304 |
Heller's Medium |
|||
| CP014-010 | GenDepot | 10X1L | EUR 99 |
Heller's Medium |
|||
| CP014-500 | GenDepot | 50L | EUR 126 |
Hoagland's Medium |
|||
| CP015-010 | GenDepot | 10X1L | EUR 99 |
Hoagland's Medium |
|||
| CP015-500 | GenDepot | 50L | EUR 126 |
Advanced Medium |
|||
| C0003-04 | Addexbio | RT 500 mL Bottle | EUR 103 |
Medium 199 |
|||
| C0012-01 | Addexbio | RT 500 mL Bottle | EUR 105 |
DC MEDIUM |
|||
| D04-117-10kg | Alphabiosciences | 10 kg | EUR 1579 |
DC MEDIUM |
|||
| D04-117-2kg | Alphabiosciences | 2kg | EUR 382 |
DC MEDIUM |
|||
| D04-117-500g | Alphabiosciences | 500 g | EUR 140 |
HLP MEDIUM |
|||
| H08-107-10kg | Alphabiosciences | 10 kg | EUR 2278 |
HLP MEDIUM |
|||
| H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 534 |
HLP MEDIUM |
|||
| H08-107-500g | Alphabiosciences | 500 g | EUR 182 |
EC MEDIUM |
|||
| E05-100-10kg | Alphabiosciences | 10 kg | EUR 814 |
EC MEDIUM |
|||
| E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 216 |
EC MEDIUM |
|||
| E05-100-500g | Alphabiosciences | 500 g | EUR 95 |
COLIFORM MEDIUM |
|||
| C03-127-10kg | Alphabiosciences | 10 kg | EUR 1324 |
COLIFORM MEDIUM |
|||
| C03-127-2kg | Alphabiosciences | 2kg | EUR 327 |
COLIFORM MEDIUM |
|||
| C03-127-500g | Alphabiosciences | 500 g | EUR 125 |
SIM MEDIUM |
|||
| S19-110-10kg | Alphabiosciences | 10 kg | EUR 1021 |
SIM MEDIUM |
|||
| S19-110-2kg | Alphabiosciences | 2kg | EUR 261 |
SIM MEDIUM |
|||
| S19-110-500g | Alphabiosciences | 500 g | EUR 107 |
SOB MEDIUM |
|||
| S19-124-10kg | Alphabiosciences | 10 kg | EUR 965 |
SOB MEDIUM |
|||
| S19-124-2kg | Alphabiosciences | 2kg | EUR 249 |
SOB MEDIUM |
|||
| S19-124-500g | Alphabiosciences | 500 g | EUR 104 |
Exosome Isolation kit (forHuman Body Fluid) |
|||
| P121 | 101Bio | - | Ask for price |
Exosome Isolation kit (forHuman Body Fluid) |
|||
| P121L | 101Bio | - | Ask for price |
Exosome Isolation kit (forHuman Body Fluid) |
|||
| P121S | 101Bio | - | Ask for price |
Cancer Antigen 125 Antigen (Ascites Fluid) |
|||
| VAng-Cr3870-10kU | Creative Biolabs | 10 kU | EUR 944 |
|
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE. |
|||
Insect Cell Medium: TNM-FH Insect Culture Medium |
|||
| ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Serum-Free Insect Culture Medium |
|||
| ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
|||
| ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
H Medium Broth |
|||
| DJ1019 | Bio Basic | 100g | EUR 60.88 |
Agarose, medium EEO |
|||
| GE4750-100G | Glentham Life Sciences | 100 g | EUR 166 |
Agarose, medium EEO |
|||
| GE4750-500G | Glentham Life Sciences | 500 g | EUR 429 |
Schwann Growth Medium |
|||
| SGM001 | Neuromics | 500 ml | EUR 244 |
Hepatocyte Maintenance Medium |
|||
| HM42600 | Neuromics | 100 ml | EUR 409 |
Neuron Growth Medium |
|||
| HNM001 | Neuromics | 500 ml | EUR 331 |
MCDB 131 Medium |
|||
| CM034-050 | GenDepot | 500ml | EUR 85 |
MCDB 131 Medium |
|||
| CM034-300 | GenDepot | 6x500ml | EUR 165 |
MCDB 131 Medium |
|||
| CM034-310 | GenDepot | 10x500ml | EUR 219 |
MCDB 131 Medium |
|||
| CM034-320 | GenDepot | 20x500ml | EUR 340 |
MCDB 131 Medium |
|||
| CM034-350 | GenDepot | 50x500ml | EUR 585 |
Williams' Medium E |
|||
| CM063-050 | GenDepot | 500ml | EUR 91 |