Improved strategies to measure finish merchandise of nitric oxide in organic fluids: nitrite, nitrate, and S-nitrosothiols.
- The purpose of this examine was to check and enhance commonplace strategies to find out nitrite (NO2-), nitrate (NO3-) and S-nitrosothiol (RSNO) ranges in cell tradition supernatants, sera, and urine. We modified the standard Griess response by changing sulfanilamide with dapsone (4,4′-diamino–diphenylsulfone) and in contrast the NO2– ranges in our examine samples with a commercially out there NO2- assay package.
- Our modification, together with ultrafiltration of the samples, resulted in an enhanced sensitivity to measure NO2- all the way down to 0.2 microM. The detection restrict was additional improved to 0.02 microM when NO2- was recognized by the fluorochrome 2,3-diaminonaphthalene (DAN). To measure the steady finish product NO3– by the Griess response or the DAN technique, this anion have to be diminished to NO2-. We in contrast the capability of bacterial nitrate reductase with the decreasing metallic cadmium to transform NO3- to NO2-.
- After discount, NO2- ranges have been decided both by the DAN technique or by our modified Griess response. We discovered that there was a excessive correlation (r2 = 0.998) in complete NO2- concentrations within the examine samples utilizing each strategies for decreasing NO3- to NO2-. The simultaneous dedication of NO2- and NO3- was achieved through the use of anion-exchange chromatography (HPLC; Polyspher IC AN-1 column).
Ferret IgG (Control, non-immune, isotype control), semi-pure for ELISA
- The detection restrict of this assay for every anion is 0.5 microM, and it may be utilized equally nicely to sera, urine, and tradition media. We additionally tailored the DAN technique to find out RSNO ranges in our examine samples. Utilizing this strategy, we have been in a position to measure RSNO ranges all the way down to 0.15 microM.
- As outcome we found that RSNO ranges have been markedly elevated in urine from septic sufferers and in supernatants from cytokine-stimulated human tumor cell traces. L-Citrulline, a coproduct of NO biosynthesis, was measured utilizing a colorimetric assay with a sensitivity restrict of three.Zero microM. Elevated L-citrulline ranges in media from traditiond cells, however not in sera or urine, correlated with elevated NO manufacturing.
Goat IgG (-ve control for flow cytometry) (isotype control)
- Though all strategies studied have been appropriate for quantifying finish merchandise of NO in organic fluids and media, using bacterial reductase and the modified Griess response proved profitable to supply the best sensitivity and linear vary for routine measurements of NO2- and NO3-.
SODIUM THIOGLYCOLLATE |
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S19-136-500g | Alphabiosciences | 500 g | Ask for price |
Thioglycollate U.S.P. |
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MED1126 | Scientific Laboratory Supplies | PK50 | EUR 45.6 |
ALT. THIOGLYCOLLATE MED. |
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A01-103-10kg | Alphabiosciences | 10 kg | Ask for price |
ALT. THIOGLYCOLLATE MED. |
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A01-103-2Kg | Alphabiosciences | 2 Kg | Ask for price |
ALT. THIOGLYCOLLATE MED. |
|||
A01-103-500g | Alphabiosciences | 500 g | Ask for price |
Bouin's Fluid |
|||
BNF125 | ScyTek Laboratories | 125 ml | EUR 75.6 |
Bouin's Fluid |
|||
BNF3800 | ScyTek Laboratories | 1 Gal. | EUR 213.6 |
Bouin's Fluid |
|||
BNF500 | ScyTek Laboratories | 500 ml | EUR 96 |
Bouin's Fluid |
|||
BNF999 | ScyTek Laboratories | 1000 ml | EUR 117.6 |
Pereneys Fluid |
|||
PFLH | Scientific Laboratory Supplies | 500ML | EUR 145.92 |
Michel's Transport Fluid |
|||
MTF-10000 | ScyTek Laboratories | 10 L | EUR 914.4 |
Michel's Transport Fluid |
|||
MTF-20000 | ScyTek Laboratories | 20 L | EUR 1608 |
Michel's Transport Fluid |
|||
MTF500 | ScyTek Laboratories | 500 ml | EUR 117.6 |
Michel's Transport Fluid |
|||
MTF999 | ScyTek Laboratories | 1000 ml | EUR 152.4 |
MagSi-DNA Body Fluid |
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MDKT00140096 | AMSBIO | 96 preps | EUR 222 |
MagSi-DNA Body Fluid |
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MDKT00140960 | AMSBIO | 10 x 96 preps | EUR 1644 |
HE4, Human ascites fluid |
|||
P1438-100 | Biovision | EUR 744 |
HE4, Human ascites fluid |
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P1438-500 | Biovision | EUR 3406.8 |
SOF Synthetic Oviduct Fluid. |
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IVL05-100ML | Caisson Labs | 100 ml | EUR 93.6 |
Description: Synthetic Oviduct Fluid. |
SOF Synthetic Oviduct Fluid. |
|||
IVL05-6X100ML | Caisson Labs | 6 x 100 ml | EUR 181.2 |
Description: Synthetic Oviduct Fluid. |
G bath fluid 5L |
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BAT8171 | Scientific Laboratory Supplies | EACH | EUR 156.18 |
G bath fluid 10L |
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BAT8172 | Scientific Laboratory Supplies | EACH | EUR 304.38 |
M bath fluid 10L |
|||
BAT8174 | Scientific Laboratory Supplies | EACH | EUR 615.6 |
HS bath fluid 10L |
|||
BAT8176 | Scientific Laboratory Supplies | EACH | EUR 820.8 |
H10 bath fluid 10L |
|||
BAT8178 | Scientific Laboratory Supplies | EACH | EUR 1023.72 |
H20S bath fluid 10L |
|||
BAT8180 | Scientific Laboratory Supplies | EACH | EUR 1053.36 |
H5 bath fluid 10L |
|||
BAT8182 | Scientific Laboratory Supplies | EACH | EUR 1038.54 |
Fluid dispenser 120cc pink |
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INS2200 | Scientific Laboratory Supplies | EACH | EUR 19.72 |
Fluid dispenser 120cc antisplash |
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INS2202 | Scientific Laboratory Supplies | EACH | EUR 20.75 |
Fluid dispenser 180cc pink |
|||
INS2204 | Scientific Laboratory Supplies | EACH | EUR 19.72 |
Fluid dispenser 180cc antisplash |
|||
INS2206 | Scientific Laboratory Supplies | EACH | EUR 13.11 |
Calibration Fluid Sucrose 60% |
|||
SS60 | Scientific Laboratory Supplies | EACH | EUR 79.8 |
CA-125, Human Ascites Fluid |
|||
P1446-10 | Biovision | EUR 352.8 |
20 x Synovial Fluid Solution |
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GR103059 | Genorise Scientific | 100 mL | EUR 84 |
Immobiline Drystrip cover fluid 1000mL |
|||
17133501 | Scientific Laboratory Supplies | EACH | EUR 95.76 |
Mounting Medium |
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4300 | Virostat | 3 ml | EUR 216.6 |
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein. |
DC MEDIUM |
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D04-117-10kg | Alphabiosciences | 10 kg | EUR 1894.8 |
DC MEDIUM |
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D04-117-2kg | Alphabiosciences | 2kg | EUR 458.4 |
DC MEDIUM |
|||
D04-117-500g | Alphabiosciences | 500 g | EUR 168 |
Advanced Medium |
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C0003-04 | Addexbio | RT 500 mL Bottle | EUR 123.6 |
Medium 199 |
|||
C0012-01 | Addexbio | RT 500 mL Bottle | EUR 126 |
COLIFORM MEDIUM |
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C03-127-10kg | Alphabiosciences | 10 kg | EUR 1588.8 |
COLIFORM MEDIUM |
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C03-127-2kg | Alphabiosciences | 2kg | EUR 392.4 |
COLIFORM MEDIUM |
|||
C03-127-500g | Alphabiosciences | 500 g | EUR 150 |
Heller's Medium |
|||
CP014-010 | GenDepot | 10X1L | EUR 118.8 |
Heller's Medium |
|||
CP014-500 | GenDepot | 50L | EUR 151.2 |
Hoagland's Medium |
|||
CP015-010 | GenDepot | 10X1L | EUR 118.8 |
Hoagland's Medium |
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CP015-500 | GenDepot | 50L | EUR 151.2 |
A Medium |
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DJ1018 | Bio Basic | 100g | EUR 101.76 |
EC MEDIUM |
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E05-100-10kg | Alphabiosciences | 10 kg | EUR 976.8 |
EC MEDIUM |
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E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 259.2 |
EC MEDIUM |
|||
E05-100-500g | Alphabiosciences | 500 g | EUR 114 |
NeuroProgenitor Medium |
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NM42400 | Neuromics | 125 ml | EUR 364.8 |
HLP MEDIUM |
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H08-107-10kg | Alphabiosciences | 10 kg | EUR 2733.6 |
HLP MEDIUM |
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H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 640.8 |
HLP MEDIUM |
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H08-107-500g | Alphabiosciences | 500 g | EUR 218.4 |
M9 Medium |
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SD7024 | Bio Basic | 250g | EUR 86.1 |
M9CA Medium |
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SD7025 | Bio Basic | 250g | EUR 86.1 |
YM Medium |
|||
SD7031 | Bio Basic | 250g | EUR 84.54 |
TYGPN Medium |
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SD7032 | Bio Basic | 250g | EUR 84.01 |
M63 Medium |
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SD7033 | Bio Basic | 250g | EUR 86.1 |
SIM MEDIUM |
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S19-110-10kg | Alphabiosciences | 10 kg | EUR 1225.2 |
SIM MEDIUM |
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S19-110-2kg | Alphabiosciences | 2kg | EUR 313.2 |
SIM MEDIUM |
|||
S19-110-500g | Alphabiosciences | 500 g | EUR 128.4 |
SOB MEDIUM |
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S19-124-10kg | Alphabiosciences | 10 kg | EUR 1158 |
SOB MEDIUM |
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S19-124-2kg | Alphabiosciences | 2kg | EUR 298.8 |
SOB MEDIUM |
|||
S19-124-500g | Alphabiosciences | 500 g | EUR 124.8 |
TCBS Medium |
|||
MED1188 | Scientific Laboratory Supplies | PK10 | EUR 7.52 |
DNase Medium |
|||
MED1196 | Scientific Laboratory Supplies | PK10 | EUR 7.98 |
OF Medium |
|||
MED1664 | Scientific Laboratory Supplies | 500G | EUR 68.4 |
Exosome Isolation kit (forHuman Body Fluid) |
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P121 | 101Bio | - | Ask for price |
Exosome Isolation kit (forHuman Body Fluid) |
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P121L | 101Bio | - | Ask for price |
Exosome Isolation kit (forHuman Body Fluid) |
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P121S | 101Bio | - | Ask for price |
BD FACS Flow Sheath Fluid 20L |
|||
342003 | Scientific Laboratory Supplies | EACH | EUR 78.66 |
Cancer Antigen 125 Antigen (Ascites Fluid) |
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VAng-Cr3870-10kU | Creative Biolabs | 10 kU | EUR 1132.8 |
Description: Cancer Antigen 125 Antigen, Host/Source: Ascites Fluid. The purity is ≥ 95% by SDS-PAGE. |
Cryoscope Heat Transfer Fluid Dairy Testing |
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HTF250 | Scientific Laboratory Supplies | 250ML | EUR 36.48 |
Insect Cell Medium: TNM-FH Insect Culture Medium |
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ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Serum-Free Insect Culture Medium |
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ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
|||
ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
A-1 MEDIUM |
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A01-116-10kg | Alphabiosciences | 10 kg | EUR 1240.8 |
A-1 MEDIUM |
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A01-116-2Kg | Alphabiosciences | 2 Kg | EUR 316.8 |
A-1 MEDIUM |
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A01-116-500g | Alphabiosciences | 500 g | EUR 129.6 |
SuperMOUNT? Mounting Medium |
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1211-20 | Biovision | EUR 241.2 |
Recovery Medium - 6x12mL |
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1711 | Intact Genomics | 4/PK | EUR 153.6 |
RPMI 1640 Medium |
|||
abx098879-500ml | Abbexa | 500 ml | EUR 210 |
Aqueous Mounting Medium |
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AR1018 | BosterBio | 10mL X5 (Enough for 800-1200 assays) | EUR 96 |
Antifade Mounting Medium |
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AR1109 | BosterBio | 10mL (for 1000 assays) | EUR 78 |
MCDB 131 Medium |
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CM034-050 | GenDepot | 500ml | EUR 102 |
MCDB 131 Medium |
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CM034-300 | GenDepot | 6x500ml | EUR 198 |
Monitoring of the uptake and metabolism of aminooxy analogues of polyamines in cultured cells by high-performance liquid chromatography.
- A high-performance liquid chromatographic technique for the dedication of polyamines and their aminooxy analogues is described. Oxime derivatization with a ketone is used to guard the aminooxy group throughout post-column response with o-phthalaldehyde. The quantity of the polyamines and of the oximes of their aminooxy analogues might be decided concurrently in traditiond cells and cell tradition media.
- The restrict of detection is 20-30 pmol, and the response of the fluorescence detection is linear as much as Four nmol. The separation of the aminooxy analogues from the naturally occurring polyamines might be various through the use of totally different ketones for oxime formation. The strategy was used to measure the soundness of aminooxy analogues of putrescine (1-aminooxy-3-aminopropane) and spermidine [N-(2-aminooxyethyl)-1,4-diaminobutane and 1-aminooxy-3-N-(3-aminopropyl)aminopropane] in cell tradition media and the uptake into traditiond child hamster kidney (BHK21/C13) cells.
Saccharomyces Cerevisiae Host Cell Proteins positive control (10 ug/ml)
- The prognosis of human herpesvirus 6 (HHV-6) an infection represents a posh difficulty as a result of essentially the most broadly used diagnostic instruments, resembling immunoglobulin G antibody titer dedication and qualitative DNA PCR with blood cells, are unable to tell apart between latent (clinically silent) and lively (usually clinically related) an infection.
Llama IgG (Control, non-immune, isotype control, ELISA grade)
- We’ve developed a brand new, extremely delicate, quantitative PCR assay for the correct measurement of HHV-6 DNA in tissue-derived cell suspensions and physique fluids. The check makes use of a 5′ nuclease, fluorogenic assay mixed with real-time detection of PCR amplification merchandise with the ABI PRISM 7700 sequence detector system. The sensitivity of this technique is the same as the sensitivity of a nested PCR protocol (decrease detection restrict,
FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-2kg | Alphabiosciences | 2kg | EUR 253.2 |
FLUID THIOGLYCOLLATE MEDIUM |
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F06-101-500g | Alphabiosciences | 500 g | EUR 112.8 |
Fluid Thiogly Medium (USP) |
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MED1472 | Scientific Laboratory Supplies | EACH | EUR 64.98 |
Fluid Thio Medium 500gm |
|||
225650 | Scientific Laboratory Supplies | EACH | EUR 117.42 |
Sabouraud Agar |
|||
MED1052 | Scientific Laboratory Supplies | PK50 | EUR 43.55 |
SABOURAUD DEXTROSE AGAR |
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S19-100-10kg | Alphabiosciences | 10 kg | EUR 900 |
SABOURAUD DEXTROSE AGAR |
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S19-100-2Kg | Alphabiosciences | 2 Kg | EUR 242.4 |
SABOURAUD DEXTROSE AGAR |
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S19-100-500g | Alphabiosciences | 500 g | EUR 109.2 |
SABOURAUD DEXTROSE BROTH |
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S19-129-10kg | Alphabiosciences | 10 kg | EUR 1216.8 |
SABOURAUD DEXTROSE BROTH |
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S19-129-2kg | Alphabiosciences | 2kg | EUR 312 |
SABOURAUD DEXTROSE BROTH |
|||
S19-129-500g | Alphabiosciences | 500 g | EUR 128.4 |
Sabouraud Dextrose Agar |
|||
MED1184 | Scientific Laboratory Supplies | PK10 | EUR 6.84 |
Sabouraud Dextrose Agar |
|||
MED1326 | Scientific Laboratory Supplies | EACH | EUR 42.86 |
Bouin's Fluid |
|||
BNF125 | ScyTek Laboratories | 125 ml | EUR 75.6 |
Bouin's Fluid |
|||
BNF3800 | ScyTek Laboratories | 1 Gal. | EUR 213.6 |
Bouin's Fluid |
|||
BNF500 | ScyTek Laboratories | 500 ml | EUR 96 |
Bouin's Fluid |
|||
BNF999 | ScyTek Laboratories | 1000 ml | EUR 117.6 |
Pereneys Fluid |
|||
PFLH | Scientific Laboratory Supplies | 500ML | EUR 145.92 |
SABOURAUD DEXTROSE AGAR, EMMONS |
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S19-102-10kg | Alphabiosciences | 10 kg | EUR 1377.6 |
SABOURAUD DEXTROSE AGAR, EMMONS |
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S19-102-2kg | Alphabiosciences | 2kg | EUR 346.8 |
SABOURAUD DEXTROSE AGAR, EMMONS |
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S19-102-500g | Alphabiosciences | 500 g | EUR 138 |
Sabouraud Dex Broth 500gm |
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238230 | Scientific Laboratory Supplies | EACH | EUR 110.58 |
Sabouraud Ag Mod 500gm |
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274720 | Scientific Laboratory Supplies | EACH | EUR 143.64 |
SABOURAUD DEXTROSE AGAR W/CHLORAMPHENICOL |
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S19-100C-10kg | Alphabiosciences | 10 kg | EUR 1224 |
SABOURAUD DEXTROSE AGAR W/CHLORAMPHENICOL |
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S19-100C-2kg | Alphabiosciences | 2kg | EUR 313.2 |
SABOURAUD DEXTROSE AGAR W/CHLORAMPHENICOL |
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S19-100C-500g | Alphabiosciences | 500 g | EUR 128.4 |
Michel's Transport Fluid |
|||
MTF-10000 | ScyTek Laboratories | 10 L | EUR 914.4 |
Michel's Transport Fluid |
|||
MTF-20000 | ScyTek Laboratories | 20 L | EUR 1608 |
Michel's Transport Fluid |
|||
MTF500 | ScyTek Laboratories | 500 ml | EUR 117.6 |
Michel's Transport Fluid |
|||
MTF999 | ScyTek Laboratories | 1000 ml | EUR 152.4 |
HE4, Human ascites fluid |
|||
P1438-100 | Biovision | EUR 744 |
HE4, Human ascites fluid |
|||
P1438-500 | Biovision | EUR 3406.8 |
MagSi-DNA Body Fluid |
|||
MDKT00140096 | AMSBIO | 96 preps | EUR 222 |
MagSi-DNA Body Fluid |
|||
MDKT00140960 | AMSBIO | 10 x 96 preps | EUR 1644 |
SOF Synthetic Oviduct Fluid. |
|||
IVL05-100ML | Caisson Labs | 100 ml | EUR 93.6 |
Description: Synthetic Oviduct Fluid. |
SOF Synthetic Oviduct Fluid. |
|||
IVL05-6X100ML | Caisson Labs | 6 x 100 ml | EUR 181.2 |
Description: Synthetic Oviduct Fluid. |
Fluid dispenser 120cc pink |
|||
INS2200 | Scientific Laboratory Supplies | EACH | EUR 19.72 |
Fluid dispenser 120cc antisplash |
|||
INS2202 | Scientific Laboratory Supplies | EACH | EUR 20.75 |
Fluid dispenser 180cc pink |
|||
INS2204 | Scientific Laboratory Supplies | EACH | EUR 19.72 |
Fluid dispenser 180cc antisplash |
|||
INS2206 | Scientific Laboratory Supplies | EACH | EUR 13.11 |
G bath fluid 5L |
|||
BAT8171 | Scientific Laboratory Supplies | EACH | EUR 156.18 |
G bath fluid 10L |
|||
BAT8172 | Scientific Laboratory Supplies | EACH | EUR 304.38 |
M bath fluid 10L |
|||
BAT8174 | Scientific Laboratory Supplies | EACH | EUR 615.6 |
HS bath fluid 10L |
|||
BAT8176 | Scientific Laboratory Supplies | EACH | EUR 820.8 |
H10 bath fluid 10L |
|||
BAT8178 | Scientific Laboratory Supplies | EACH | EUR 1023.72 |
H20S bath fluid 10L |
|||
BAT8180 | Scientific Laboratory Supplies | EACH | EUR 1053.36 |
H5 bath fluid 10L |
|||
BAT8182 | Scientific Laboratory Supplies | EACH | EUR 1038.54 |
Calibration Fluid Sucrose 60% |
|||
SS60 | Scientific Laboratory Supplies | EACH | EUR 79.8 |
CA-125, Human Ascites Fluid |
|||
P1446-10 | Biovision | EUR 352.8 |
20 x Synovial Fluid Solution |
|||
GR103059 | Genorise Scientific | 100 mL | EUR 84 |
Immobiline Drystrip cover fluid 1000mL |
|||
17133501 | Scientific Laboratory Supplies | EACH | EUR 95.76 |
A Medium |
|||
DJ1018 | Bio Basic | 100g | EUR 101.76 |
DC MEDIUM |
|||
D04-117-10kg | Alphabiosciences | 10 kg | EUR 1894.8 |
DC MEDIUM |
|||
D04-117-2kg | Alphabiosciences | 2kg | EUR 458.4 |
DC MEDIUM |
|||
D04-117-500g | Alphabiosciences | 500 g | EUR 168 |
Advanced Medium |
|||
C0003-04 | Addexbio | RT 500 mL Bottle | EUR 123.6 |
Medium 199 |
|||
C0012-01 | Addexbio | RT 500 mL Bottle | EUR 126 |
COLIFORM MEDIUM |
|||
C03-127-10kg | Alphabiosciences | 10 kg | EUR 1588.8 |
COLIFORM MEDIUM |
|||
C03-127-2kg | Alphabiosciences | 2kg | EUR 392.4 |
COLIFORM MEDIUM |
|||
C03-127-500g | Alphabiosciences | 500 g | EUR 150 |
Heller's Medium |
|||
CP014-010 | GenDepot | 10X1L | EUR 118.8 |
Heller's Medium |
|||
CP014-500 | GenDepot | 50L | EUR 151.2 |
Hoagland's Medium |
|||
CP015-010 | GenDepot | 10X1L | EUR 118.8 |
Hoagland's Medium |
|||
CP015-500 | GenDepot | 50L | EUR 151.2 |
EC MEDIUM |
|||
E05-100-10kg | Alphabiosciences | 10 kg | EUR 976.8 |
EC MEDIUM |
|||
E05-100-2Kg | Alphabiosciences | 2 Kg | EUR 259.2 |
EC MEDIUM |
|||
E05-100-500g | Alphabiosciences | 500 g | EUR 114 |
Mounting Medium |
|||
4300 | Virostat | 3 ml | EUR 216.6 |
Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein. |
NeuroProgenitor Medium |
|||
NM42400 | Neuromics | 125 ml | EUR 364.8 |
HLP MEDIUM |
|||
H08-107-10kg | Alphabiosciences | 10 kg | EUR 2733.6 |
HLP MEDIUM |
|||
H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 640.8 |
HLP MEDIUM |
|||
H08-107-500g | Alphabiosciences | 500 g | EUR 218.4 |
SIM MEDIUM |
|||
S19-110-10kg | Alphabiosciences | 10 kg | EUR 1225.2 |
SIM MEDIUM |
|||
S19-110-2kg | Alphabiosciences | 2kg | EUR 313.2 |
SIM MEDIUM |
|||
S19-110-500g | Alphabiosciences | 500 g | EUR 128.4 |
SOB MEDIUM |
|||
S19-124-10kg | Alphabiosciences | 10 kg | EUR 1158 |
SOB MEDIUM |
|||
S19-124-2kg | Alphabiosciences | 2kg | EUR 298.8 |
SOB MEDIUM |
|||
S19-124-500g | Alphabiosciences | 500 g | EUR 124.8 |
M9 Medium |
|||
SD7024 | Bio Basic | 250g | EUR 86.1 |